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20150915 NL GUTS Presentations Affinity Separation

Last updated 864 days ago by Albert Verver

Group of Users of Technology for Separation in the Netherlands





20150915 Wageningen University - Mrs. Marta Rodriguez Illara –“Structural Adsorbents (Monoliths)”

Monoliths in chromatography are defined as ‘one piece stationary phase with (micro)channels. The channels are random or structured (channeled). Applying channel monoliths to isolate functional food ingredients requires understanding how they work. Findings from models and experiments are that polymeric columns have low surface area for small molecules, cell density not sufficient, high axial dispersion, early breakthrough. On the other hand they have a higher specific permeability, low plugging, low pressure drop. With the right design method, channel monoliths need lower resin volumes, specific productivity is 2,5 time higher, better at low K-values, but design challenges are still: smaller channel diameters, thinner walls, more efficient geometries. Low pressure drop allows long columns or in series. An invitation for monolith manufacturers to develop and produce monoliths for food industry. http://library.wur.nl/WebQuery/clc/2070173


20150915 Technical University Delft - Mrs. Silvia Pirrung-“Affinity separations by process modelling/ synthesis”

The Bioprocess Engineering group of TUD works on process modelling and process synthesis for affinity separations and specifically high throughput downstream processes defining the ‘best’ combination of unit operations to isolate and purify a compound. Starting from chemical and physical properties, process and equipment boundaries, the PSE exercise is reduced by importing experimental chromatographic data, generated specifically for this purpose, into the process modeling. Good correspondence between predicted and experimental values supports sizing of the chromatography equipment and economic evaluation of a set of options, defined with input of professionals expertise. The approach was illustrated with a case study and seems applicable for other unit operations. http://www.tnw.tudelft.nl/nl/over-faculteit/afdelingen/biotechnology/research-groups/bioprocess-engineering/


20150915 Proxcys B.V. – Mr. Thijs Groenewegen & Bas Stevens – “Fishing proteins from blood plasma”

With its Radial Low Chromatography RFC technology the SME Proxcys has grown to international presence. The RFC is compact, suitable for high throughput lab and large, tolerant for process errors and allows crude feeds with quite some impurities. Applications are inbio-pharmaceuticals, functional food ingredients, direct capture, blood-plasma protein fractionation. For the latter RLC is crucial in the one-line process of several RLC columns in series, capable of recovering at least 6 valuable proteins from one batch of native blood-plasma without the use of ethanol as a solvent and overcoming sanitary risks and energy consumptions of current time consuming practice.
The same robustness for crude feeds enables the fast direct capture of IgC from high density CHO-S cells with very high purity after two washes. www.proxcys.com


20150915 EColoRO B.V.- Mr. Eric van Sonsbeek & Han Oude Groeniger - “Zero discharge with large scale Electrocoagulation: First experience results and upcoming European demonstration”

From solving major operating problems of an ineffective textile wastewater treatment plant WWTP of 200 m³/h the founders of the start-up EColoRO developed a novel system to recover 90% of the water and remove 93-96% of colour, by adsorption to freshly generated iron-flocs, at acceptable investment and running cost. Heart of the system is a revised Electro-Coagulation reactor in combination with DAF/UF operated in a novel way followed by RO/NF. EC sludge, mainly iron-oxide/hydroxide is much less than for conventional bio and physical-chemical treatment. DS is >50% after filter press. The power uptake is around 1 kWh/m³. Total running cost is below fresh water intake. Higher water recoveries can be achieved with an evaporator. The concept received a H2020 grant through ISPT to develop a pilot and demo-plant for several locations in EU. www.ecoloro.nl  www.ecwrti.eu  www.ispt.eu


20150915 Wageningen University B.V. – Mrs. Lena Jankowiak – “Isolations of Isoflavones from Okara”

This directly transmitted Webinar from Chili presents the development at WUR of a sustainable non-toxic solvent process to recover isoflavones from Okara a byproduct of soymilk. Okara is rich in dietary fiber, proteins, vegetable oils and food ingredients, amongst which 0,1%. Isoflavones comprising 4 groups of each 3 beneficial compounds. From the physical properties of the compounds, the best approach was extraction combined with a modified adsorbent. The non-toxic solvent of choice appeared not to be the best in a combined system, because it has to function more as a carrier. Combined A/E however increases yield, while allowing a drastic decrease of liquid-solid ratio. Implementing the technology is feasible. https://www.wageningenur.nl/en/Publication-details.htm?publicationId=publication-way-343536333533


20150915 Synthon B.V. - Mr. Michel Eppink – ”Affinity separations of bio-therapeutic proteins”

Currently one of the larger medium sized pharmaceutical companies in NL. Synthons focus is oncology and autoimmune diseases in ultra-modern production facilities with tendency to disposable reactors where feasible. Synthons programs cover cell killing monoclonal anti-bodies, harvested, extracted, fractionated and purified with functionalized membranes, beads, precipitated or crystallised according to in-house developed DSP trains. Chromatographic techniques used are GPC, IEX, HIC, HAP, RPC while Bio-affinity AC was discussed in more detail. Bio-AC works on specific interaction between an anti-body and an anti-gen. Enriched by elution, purities of >95% can be reached in one step. Synthon typically follows the feasibility lab stage pilot stage sequence, but is able to work rapidly thanks to an automised technology screening platform, high throughput analysis and test methodology. Selected resins are studied in more detail for scale-up to process level. Examples were elucidated in detail. www.synthon.com


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Visit the NL GUTS website www.processinnovation.nl/nlguts
For more information about NL GUTS please contact:
Jan Koning (chairman NL GUTS)
tel +32 473 976 135 - koning@trionpartners.nl
Bart Manders  (contact at RVO)
tel +31(0)88 602 2329 - bart.manders@rvo.nl

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